Enterotoxin-producing strains of Bacillus thuringiensis isolated from food |
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| Authors: | PH Damgaard HD Larsen BM Hansen J Bresciani K Jørgensen |
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| Institution: | Department of Ecology and Molecular Biology, Section os Zoology;Department of Veterinary Microbiology, The Roaly Veterinary and Agricultural University, Frederiksberg;National Environmental Research Institute, Roskilde, Denmark |
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| Abstract: | P.H. DAMGAARD, H.D. LARSEN, B.M. HANSEN, J. BRESCIANI AND K. JØRGENSEN. 1996. Strains of Bacillus thuringiensis were isolated from various food items (pasta, pitta bread and milk) and were found to belong to either H-serotype kurstaki or neoleonensis. The strains were bioassayed against Pieris brassicae and insecticidal activity of strains was found to correspond to the presence of the cry1.A -gene. All strains, except one, were found to express cytotoxic effects on Vero cells as an indicator of enterotoxin activity. Further, the B. thuringiensis strains HD-1 (serotype kurstuki ), NB-125 (serotype tenebrionis ) and HD-567 (serotype isruelensis ) which are used commercially for insect pest management, were also found to have cytotoxic effects on Vero cells. |
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