The application of new aminoorganic silanes for affinity chromatography of plant peroxidases on vanillin-liganded controlled porosity glass columns

In this work, new packings consisting of home made controlled porosity glass, new self-prepared aminoorganic silanes and vanillin as a ligand were used for affinity chromatography of some plant peroxidases (donor: H₂O₂ oxidoreductases; EC 1.11.1.7). The procedure allowed simple and relatively high purification of enzymes. The results were compared with those obtained with the material including commercially available γ-aminopropyltriethoxysilane.