Inorganic carbon acquisition in Riccia fluitans L |
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Authors: | Ballesteros D; Garcia-Sanchez M; Heredia M; Felle H; Fernandez J |
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Institution: | Departmento de Biologia Vegetal, Facultad de Ciencias, Universidad de Malaga, Campus de Teatinos s/n, 29071 Malaga, Spain; Botanisches Institut der Justus von Liebig Universitat, Giessen, Germany; Corresponding author |
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Abstract: | The response of photosynthetic rate to pH indicates that CO2 is the
inorganic carbon (Ci) species preferentially used by the liverwort
Riccia fluitans for photosynthesis. The absence of
external carbonic anhydrase (CA) activity and insensitivity to the
anion-exchanger inhibitor
4,4-diisothiocyanostilbene-2,2-disulphonate (DIDS)
suggest that bicarbonate is not taken up. Cultivation with bicarbonate
produces a decrease in the semi-saturation constant for
Ci and an increase in soluble CA activity, but maximum
photosynthetic rate decreases and no significant change in the Ci
compensation point occurs. Plants cultivated at 1% CO2 show no significant
differences in photosynthetic characteristics and CA activity from control
plants. Electrophysiological measurements also suggest that CO2 is the form
that crosses the plasmalemma. Application of 1% CO2 results in a transient
hyperpolarization of the membrane potential (Em) and
also a transient acidification of the cytoplasmic pH (pHc). Addition of 1
mM bicarbonate at pH 7.3 produces a similar but less marked response; at an
external pH of 8.3 no acidification is observed. These results suggest that
bicarbonate is not transported, because its effect mimics the response
caused by CO2 which enters the cell inducing a fall in cell pH, and a
hyperpolarization of Em probably due to stimulation of
the proton pump.Key words: Riccia fluitans, inorganic
carbon, membrane potential, cytoplasmic pH, photosynthesis.
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