Estimating the variation in S phase duration from flow cytometric histograms |
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Authors: | Larsson Sara Ryden Tobias Holst Ulla Oredsson Stina Johansson Maria |
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Institution: | Centre for Mathematical Sciences, Division of Mathematical Statistics, Lund University, P.O. Box 118, 221 00 Lund, Sweden. sara@maths.lth.se |
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Abstract: | A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data. |
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Keywords: | Branching process Cell cycle progression S phase duration Flow cytometry |
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