Human mesenchymal stem cells and renal tubular epithelial cells differentially influence monocyte-derived dendritic cell differentiation and maturation |
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| Authors: | Kronsteiner Barbara Peterbauer-Scherb Anja Grillari-Voglauer Regina Redl Heinz Gabriel Christian van Griensven Martijn Wolbank Susanne |
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| Institution: | aRed Cross Blood Transfusion Service of Upper Austria, Krankenhausstrasse 7, A-4017 Linz, Austria;bLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstrasse 13, A-1200 Vienna, Austria;cAustrian Cluster for Tissue Regeneration, Austria;dInstitute for Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria |
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| Abstract: | Mesenchymal stem cells (MSCs) possess immunosuppressive properties. But also fully differentiated human renal tubular epithelial cells (RTECs) are able to modulate T-cell proliferation in vitro. In this study we compared two MSC populations, human adipose derived stem cells (ASCs) and human amniotic mesenchymal stromal cells (hAMSCs), and RTECs regarding their potential to inhibit monocyte-derived dendritic cell (DC) differentiation and maturation in indirect co-culture.In the presence of hAMSCs and RTECs, monocytes stimulated to undergo DC differentiation were inhibited to acquire surface phenotype of immature and mature DCs. In contrast, ASCs showed only limited suppressive capacity. Secretion of IL-12p70 was suppressed in hAMSC co-cultures and high IL-10 levels were detected in all co-cultures. Prostaglandin E2 was found in ASC and hAMSC co-cultures, whereas soluble human leukocyte antigen-G was highly elevated only in RTEC co-cultures. Thus, inhibition of DC generation by MSCs and RTECs might be mediated by different soluble factors. |
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| Keywords: | Mesenchymal stem cells Renal tubular epithelial cells Dendritic cells Immunomodulation |
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