Soluble granzyme B and cytotoxic T lymphocyte activity in the pathogenesis of systemic lupus erythematosus |
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Authors: | Shah Dilip Kiran Ravi Wanchu Ajay Bhatnagar Archana |
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Institution: | aDepartment of Biochemistry, Basic Medical Sciences Block, Panjab University, Chandigarh, India;bDivision of Arthritis and Rheumatic Diseases-OP09, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA |
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Abstract: | Activated cytotoxic T lymphocyte (CTL) mediated target cell death has been implicated in the development of systemic autoimmune disease like SLE. However, the role of soluble granzyme B and its relationship with CTL activity and disease activity is still unknown. In this study, we evaluated role of soluble granzyme B and cytotoxic T lymphocyte activity in SLE patients. The soluble granzyme B was measured in the serum by an enzyme-linked immunosorbent assay while cytotoxic T lymphocyte activity was measured by flow cytometry. The disease activity was determined by using SLE Disease Activity Index (SLEDAI) score. Cytotoxic T lymphocyte activity was increased and strongly associated with disease activity. The soluble granzyme B levels were higher in SLE patients and associated with various clinical features like reduced complement components; C3 & C4 and skin lesion. The soluble granzyme B levels were also sturdily related with severity of the disease. The findings of this study suggest that excessive secretion of soluble granzyme B and enhanced activity of cytotoxic T lymphocyte may play a vital role in the pathogenesis of SLE and organ damage. Also, evaluation of soluble granzyme B may be helpful in monitoring the clinical features associated with activated CTL in SLE. |
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Keywords: | Soluble granzyme B Cytotoxic T lymphocyte SLEDAI score Systemic lupus erythematosus |
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