The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method |
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Authors: | Veselá Romana Doležalová Ludmila Pytlík Robert Rychtrmocová Hana Marečková Helena Trněný Marek |
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Institution: | a1st Department of Medicine, 1st Faculty of Medicine and General University Hospital, Charles University, U Nemocnice 2, 128 08 Praha, Czech Republic;bInstitute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Praha, Czech Republic;cInstitute of Immunology and Microbiology, 1st Faculty of Medicine and General University Hospital, Charles University, Studni?kova 7, 128 00 Praha, Czech Republic |
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Abstract: | Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, 3H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. It is based on time limited acquisition of events and on its distribution according to forward and side scatter values. We decided to compare these two methods and determine mutual correlation and compatibility. Eleven patients were studied and in all cases DCs promoted the survival and proliferation of both CD4 and CD8 lymphocytes. Both methods retained consistency with regard to survival and proliferation of CD4/CD8 lymphocytes. However, the correlation of these methods was not convincing. Therefore, both these methods might be used for evaluation of MLR, but each of them gives specific and complementary information. |
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Keywords: | 3H-Thymidine incorporation Flow cytometry Differential gating Expression of activation marker T-Lymphocyte proliferation |
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