Overexpression of megsin induces mesangial cell proliferation and excretion of type IV collagen in vitro |
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| Authors: | Xia Yunfeng Zhang Yimin Shi Wei Liu Shuangxin Chen Yuanhan Liang Xinling Ye Zhiming |
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| Institution: | aDepartment of Nephrology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, PR China;bDepartment of Nephrology, The Second Affiliated Hospital, Sun Yat-sen University, GuangZhou, PR China |
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| Abstract: | Over-expression of megsin is associated with mesangial cell (MC) proliferation and extracellular matrix (ECM) accumulation. The underlying pathogenesis is unknown. This study demonstrate that over-expression of megsin induced incorporation of 3H]thymidine in MCs and PDGF-BB, TGF-β1 upregulation. Concentrations of PDGF-BB, TGF-β1 and type IV collagen in the culture medium of MCs transfected with megsin were higher than controls. Anti-PDGF-BB suppressed incorporation of 3H]thymidine in MCs transfected with megsin and mRNA expression of TGF-β1 in stable transformant MCs, suggesting that over-expression of megsin induces cell proliferation and ECM accumulation in MCs, upregulation of PDGF-BB and TGF-β1 is probably the main route involved in pathogenesis. |
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| Keywords: | Megsin Transfection Mesangial cell Proliferation Cytokine |
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