Inhibition of proinflammatory macrophage responses and lymphocyte proliferation in vitro by ethyl acetate leaf extract from Daphne gnidium |
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Authors: | Harizi Hedi Chaabane Fadwa Ghedira Kamel Chekir-Ghedira Leila |
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Affiliation: | aLaboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine Dentaire, Avenue Avicenne, 5019 Monastir, Tunisia;bUnité de Pharmacognosie et de Biologie Moléculaire et Cellulaire, Faculté de Pharmacie, Avenue Avicenne, 5019, Monastir, Tunisia |
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Abstract: | Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pro-inflammatory cytokines (IL-1β and TNF-α), cyclooxygenase-2-derived prostaglandinE2 (PGE2) and iNOS-II-synthesised nitric oxide (NO) were examined. EA extract effect on mitogen-induced lymphocyte proliferation was also investigated. We reported for the first time that D. gnidium EA leaf extract dose-dependently inhibits macrophage proinflammatory function by reducing LPS-induced production of IL-1β, TNF-α, COX-2-derived PGE2 and iNOS-II-synthesised NO. Mitogen-induced lymphocyte proliferation was also dose-dependently inhibited by the extract. Lectin-induced response appears to be more sensitive to the suppressive effects of the extract than LPS-stimulated response. Collectively, these results demonstrate that D. gnidium EA leaf extract acts as an in vitro anti-inflammatory factor by inhibiting mice macrophage and lymphocyte activities. |
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Keywords: | Abbreviations: EA, ethyl acetate PGE2, prostaglandin E2 TNF-α, tumor necrosis factor-α IL-1β, interleukin-1β COX-2, cyclooxygenase-2 NO, nitric oxide LPS, lipopolysaccharide |
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