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Immunomodulatory effect of human umbilical cord Wharton's jelly-derived mesenchymal stem cells on lymphocytes |
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| Authors: | Zhou Changhui Yang Bo Tian Yi Jiao Hongliang Zheng Wendi Wang Jian Guan Fangxia |
| Affiliation: | aBioengineering Department of Zhengzhou University, Zhengzhou 450001, Henan Province, China;bDepartment of Neurosurgery, First Affiliated Hospital of Zhenzhou University, Zhengzhou 450052, Henan Province, China;cDepartment of Clinical Medicine, Zhengzhou University, Zhengzhou 450001, Henan Province, China;dDepartment of Anesthesiology/Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA |
| Abstract: | Studies have shown that mesenchymal stem cells (MSCs) have low immunogenicity and immune regulation. Human umbilical cord Wharton’s jelly provides a new source for MSCs that are highly proliferative and have multi-differentiation potential. To investigate immunomodulatory effects of human Wharton’s jelly cells (WJCs) on lymphocytes, we successfully isolated MSCs from human umbilical cord Wharton’s jelly. WJCs expressed MSC markers but low levels of human leukocyte antigen (HLA)-ABC and no HLA-DR. These results indicate that WJCs have low immunogenicity. Both WJCs and their culture supernatant could inhibit the proliferation of phytohemagglutinin-stimulated human peripheral blood lymphocytes and mouse splenocytes. Additionally, WJCs suppressed secretion of transforming growth factor-β1 and interferon-γ by human peripheral blood lymphocytes. We conclude that the immunomodulatory effect of WJCs may be related to direct cell contact and inhibition of cytokine secretion by human peripheral blood lymphocytes. |
| Keywords: | Wharton&rsquo s jelly Mesenchymal stem cells Lymphocytes Immunomodulatory Interferon-γ Transforming growth factor-β1 |
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