A rapid and inexpensive labeling method for microarray gene expression analysis |
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| Authors: | Mario Ouellet Paul D Adams Jay D Keasling Aindrila Mukhopadhyay |
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| Affiliation: | (1) Lawrence Berkeley National Laboratory, The Joint Bioenergy Institute, Emeryville, USA;(2) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA;(3) Department of Chemical Engineering, University of California, Berkeley, USA;(4) Department of Bioengineering, University of California, Berkeley, USA |
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| Abstract: | Background Global gene expression profiling by DNA microarrays is an invaluable tool in biological research. However, existing labeling methods are time consuming and costly and therefore often limit the scale of microarray experiments and sample throughput. Here we introduce a new, fast, inexpensive method for direct random-primed fluorescent labeling of eukaryotic cDNA for gene expression analysis and compare the results obtained on the NimbleGen microarray platform with two other widely-used labeling methods, namely the NimbleGen-recommended double-stranded cDNA protocol and the indirect (aminoallyl) method. |
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