Unaltered D1, D2, D4, and D5 dopamine receptor mRNA expression and distribution in the spinal cord of the D3 receptor knockout mouse |
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| Authors: | Hong Zhu Stefan Clemens Michael Sawchuk Shawn Hochman |
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| Affiliation: | (1) Department of Physiology, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA;(2) Present address: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, 313 Ferst Drive, Atlanta, GA 30332, USA |
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| Abstract: | Dopamine (DA) acts through five receptor subtypes (D1–D5). We compared expression levels and distribution patterns of all DA mRNA receptors in the spinal cord of wild-type (WT) and loss of function D3 receptor knockout (D3KO) animals. D3 mRNA expression was increased in D3KO, but no D3 receptor protein was associated with cell membranes, supporting the previously reported lack of function. In contrast, mRNA expression levels and distribution patterns of D1, D2, D4, and D5 receptors were similar between WT and D3KO animals. We conclude that D3KO spinal neurons do not compensate for the loss of function of the D3 receptor with changes in the other DA receptor subtypes. This supports use of D3KO animals as a model to provide insight into D3 receptor dysfunction in the spinal cord. |
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| Keywords: | Monoamines Real-time PCR In situ hybridization Restless Legs Syndrome Dopamine |
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