| 食品中脊髓灰质炎病毒RT-PCR检测体系的建立 |
| |
| 引用本文： | 黄一,李想,潘良文,卢钟山,吕蓉,张舒亚,高琴,刘月明. 食品中脊髓灰质炎病毒RT-PCR检测体系的建立[J]. 生物技术通讯, 2010, 21(1): 65-69. DOI: 10.3969/j.issn.1009-0002.2010.01.016 |
| |
| 作者姓名： | 黄一 李想 潘良文 卢钟山 吕蓉 张舒亚 高琴 刘月明 |
| |
| 作者单位： | 1. 华东理工大学生物工程学院,上海,200237;上海出入境检验检疫局,上海,200135
2. 上海出入境检验检疫局,上海,200135 |
| |
| 基金项目： | 上海技术标准专项，国家质检总局科研项目，科技部世博科技专项 |
| |
| 摘 要： | 目的:建立食品中脊髓灰质炎病毒的RT-PCR检测体系。方法:在脊髓灰质炎病毒基因组2A区保守序列设计引物,建立同时适于3种血清型脊髓灰质炎病毒的检测方法,并对人工接种病毒的贝类(生蚝、文蛤)、蔬果(草莓、樱桃和生菜)和水样等实际样品进行检测。结果:基于构建的分别适于3种血清型脊髓灰质炎病毒检测的参照分子,确定所建立方法的检测下限可达50拷贝参照分子DNA。针对各种食品基质建立的脊髓灰质炎病毒富集、RNA提取和RT-PCR方法检测3种血清型病毒可达10RT-PCR单位(RTU)。结论:建立的RT-PCR检测体系可用于食品中脊髓灰质炎病毒的检测。
|
| 关 键 词： | 脊髓灰质炎病毒 RT-PCR 食品 参照分子 |
Detection of Polioviruses by RT-PCR in Foodstuffs |
| |
| HUANG Yi,LI Xiang,PAN Liang-Wen,LU Zhong-Shan,L Rong,ZHANG Shu-Ya,GAO Qin,LIU Yue-Ming. Detection of Polioviruses by RT-PCR in Foodstuffs[J]. Letters in Biotechnology, 2010, 21(1): 65-69. DOI: 10.3969/j.issn.1009-0002.2010.01.016 |
| |
| Authors: | HUANG Yi LI Xiang PAN Liang-Wen LU Zhong-Shan L Rong ZHANG Shu-Ya GAO Qin LIU Yue-Ming |
| |
| Affiliation: | HUANG Yi,LI Xiang,PAN Liang-Wen,LU Zhong-Shan,L(U) Rong,ZHANG Shu-Ya,GAO Qin,LIU Yue-Ming |
| |
| Abstract: | Objective:To establish a RT-PCR system suitable for polioviruses detection in foodstuffs.Methods: A primer pair based on the conserved 2A region of poliovirus genomic cDNA has been designed to set up the detection system for three serotypes of polioviruses.Moreover,several inoculated samples,including oyster,clam, strawberry,cherry,lettuce and water were analyzed by the developed detection system.Results:Sensitivity tests based on three constructed reference molecules showed that 50 copies of reference mole... |
| |
| Keywords: | RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
