Absence of the symbiont Candidatus Midichloria mitochondrii in the mitochondria of the tick Ixodes holocyclus |
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| Authors: | Tiziana Beninati,Markus Riegler,Inger-Marie E. Vilcins,Luciano Sacchi,Raelene McFadyen,Mark Krockenberger,Claudio Bandi,Scott L. O''Neill,& Nathan Lo |
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| Affiliation: | Centre for Microbial Diseases and Immunity Research, University of British Columbia, Lower Mall Research Station, Vancouver, BC, Canada;;Department of Biotechnology, Biocenter of the University of Würzburg, Würzburg, Germany;;and School of Science and Technology, Jacobs University Bremen, Bremen, Germany |
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| Abstract: | OprG of Pseudomonas aeruginosa is a member of the very large and widely distributed but poorly characterized OmpW (PF0392) family of outer membrane proteins. It was established here that OprG was highly transcribed in anaerobic environments rich in iron via the ANR regulator. In the absence of OprG, P. aeruginosa was significantly less cytotoxic toward human bronchial epithelial cells. Planar bilayer studies indicated that purified OprG formed cationic-selective channels with a conductance of 500 pS in 1 M KCl; however, contrary to previous reports, OprG did not appear to be involved in either iron or antibiotic uptake. |
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| Keywords: | Pseudomonas aeruginosa outer membrane OprG anaerobic porin |
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