Use of Flow Cytometry To Monitor Legionella Viability |
| |
| Authors: | S verine Allegra, Fran oise Berger, Philippe Berthelot, Florence Grattard, Bruno Pozzetto, Serge Riffard |
| |
| Affiliation: | Séverine Allegra, Françoise Berger, Philippe Berthelot, Florence Grattard, Bruno Pozzetto, and Serge Riffard |
| |
| Abstract: | Legionella viability was monitored during heat shock treatment at 70°C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae. |
| |
| Keywords: | |
|
|
