Effects of forskolin and cholera toxin on cyclic AMP release in a neurotensin-secreting rat C-cell line |
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Authors: | F Zeytin C Mougin P Brazeau N Ling |
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Institution: | Laboratories for Neuroendocrinology The Salk Institute for Biological Studies 10010 North Torrey Pines Road, La Jolla CA 92037 USA |
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Abstract: | The effects of forskolin and cholera toxin on the regulation of cAMP release were studied in a neurotensin-secreting rat C-cell line. The interaction of these agents with norepinephrine, a potent neurotensin secretagogue, was also investigated. Forskolin stimulated cAMP release 10(2)-10(3) fold while it increased neurotensin release 2-3 fold. Cholera toxin caused a 10(2)-10(3) fold increase in cAMP release and had no effect on neurotensin release. We conclude that the 44-2 C-cells provide a new model for studying the regulation of the concomitant (via forskolin) or independent (via cholera toxin) secretion of cyclic AMP and/or neurotensin. |
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Keywords: | NT neurotensin CT calcitonin KRBG Krebs-Ringr-bicarbonate buffer with 90 mgs% glucose pH 7 4 DMEM Dulbecco's Modified Eagle's medium supplemented with 15% horse serum NE norepinephrine RIA radioimmunoassay |
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