Xyloglucan from the leaves of Hymenaea courbaril |
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Authors: | Busato A P Vargas-Rechia C G Reicher F |
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Affiliation: | Departmento de Bioquímica, Universidade Federal do Paraná, CP 19046, 81531-990, Curitiba, Paraná, Brazil. |
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Abstract: | A fucosylated xyloglucan was isolated from the leaves of Hymenaea courbaril by alkaline extraction, followed by ethanol precipitation and ion-exchange chromatography. The isolated polysaccharide showed Glc:Xyl:Gal:Fuc in molar ratio of 8:5:2.5:1 and (D)(25) +40.5 degrees. Composition and linkage analyses, supported by NMR spectroscopic measurements, showed that the polysaccharide has a glucan backbone which is highly substituted at O-6 with D-xylopyranose residues, about a half of which are substituted at O-2 by D-galactopyranosyl units. Some of the galactose residues are further substituted by L-fucopyranose at O-2. The M(r), as determined by HPSEC, was 49,500. |
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