Induction of mutants in Saccharomyces cerevisiae by guanidine hydrochloride II. Conditions that prevent induction |
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| Authors: | Maria Helena Juliani SOP da Costa |
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| Institution: | 1. Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, C.P. 20780, Sao Paulo Brasil;7. Departemento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Sao Paulo Brasil |
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| Abstract: | The induction of − “petite” mutants by guanidine hydrochloride (GuHCl) is inhibited in several conditions. Anaerobiosis inhibited the induction either with or without cell multiplication. Both nalidixic acid (NA) and cycloheximide (CH) inhibited the induction of mutants. On the other hand, chloramphenicol (CAP) produced a dual effect: at low concentration it stimulated, at high concentration it inhibited, the induction. The effect of these different inhibitors on the transformation of + mother cells into − by GuHCl is discussed. |
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| Keywords: | CAP chloramphenicol CH cycloheximide EtBr ethidium bromide GuHCl guanidine hydrochloride NA nalidixic acid TTC triphenyl tetrazolium chloride |
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