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Autocrine activation of cultured macrophages by brain-derived neurotrophic factor
Authors:Asami Toshio  Ito Takuya  Fukumitsu Hidefumi  Nomoto Hiroshi  Furukawa Yoshiko  Furukawa Shoei
Institution:Laboratory of Molecular Biology, Gifu Pharmaceutical University, 5-6-1, Mitahora-Higashi, Gifu 502-8585, Japan.
Abstract:To elucidate a significance of the expression of brain-derived neurotrophic factor (BDNF) in the activated microglia/macrophages of the injured central nervous system, we examined BDNF actions on or BDNF synthesis by macrophages cultured from the mouse peritoneal cavity. They synthesized BDNF and neurotrophin-3 (NT-3) in addition to expressing high-affinity neurotrophin receptors, full-length TrkB (FL), truncated TrkB (TK(-)), and TrkC, thus suggesting an autocrine influence of BDNF and NT-3. BDNF, but not NT-3, enhanced phagocytic activity and stimulated synthesis/secretion of interleukin-1beta in the same manner as lipopolysaccharide (LPS). Furthermore, there was a significant correlation of the phagocytic activity with the expression of BDNF or TrkB (FL). These results imply that the phagocytic activity of macrophages depends on BDNF synthesis and/or TrkB (FL) expression, suggesting that BDNF participates in the activation processes of macrophages by acting in an autocrine manner.
Keywords:Macrophages  Culture  Phagocytic activity  Brain-derived neurotrophic factor (BDNF)  TrkB  Neurotrophin-3 (NT-3)  Lipopolysaccharide (LPS)
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