Development and applications of a yeast-based bioassay for the mycotoxin zearalenone |
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Authors: | R. Mitterbauer H. Bachmann B. Poppenberger N. Safaie G. Adam |
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Affiliation: | (1) Center of Applied Genetics, BOKU — University of Natural Resources and Applied Life Sciences, A-1190 Vienna, Austria;(2) College of Agriculture, Tarbiat Modarres University, P.O. Box 14155-4838, Tehran, Islamic Republic of Iran |
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Abstract: | Zearalenone (ZON) is a non-steroidal estrogenic mycotoxin produced by plant pathogenic species ofFusarium. As a consequence of infection withF. culmorum andF. graminearum, ZON can be found in cereals and derived food products. Several countries have established monitoring programs and guidelines for ZON levels in grain intended for human consumption and animal feed. We have developed a sensitive yeast bioassay allowing detection of the estrogenic activity of ZON in cereal extracts without requiring further clean up steps. The high sensitivity makes this assay suitable for low cost monitoring of contamination of small grain cereals with estrogenicFusarium mycotoxins, but also attractive as a tool for basic research. We have successfully used yeast indicator strains to screen for mutants ofF. graminearum which no longer produce detectable amounts of ZON, and have identified a plant cDNA encoding a ZON detoxification enzyme. Presented at the 25th Mykotoxin Workshop in Giessen, Germany, May 19–21, 2003 |
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Keywords: | zearalenone estrogenic bioassay Fusarium |
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