Ethanol induces secretion of oxidized proteins by pancreatic acinar cells |
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Authors: | V O Palmieri I Grattagliano G Palasciano |
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Institution: | (1) Section of Internal Medicine – Department of Internal and Public Medicine (DIMIMP), University Medical School of Bari, Bari, Italy;(2) Clinica Medica ‘A. Murri’, Dipartimento di Medicina Interna e Pubblica (DIMIMP), Università degli Studi di Bari, Piazza G. Cesare, 11-70124 Bari, Italy |
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Abstract: | The pancreas is vulnerable to ethanol toxicity, but the pathogenesis of alcoholic pancreatitis is not fully defined. The intracellular
oxidative balance and the characteristics of the secretion of isolated rat pancreatic acinar cells stimulated with the cholecystokinin
analogue cerulein were assayed after acute oral ethanol (4 g/kg) load. Pancreatic acinar cells from ethanol-treated rats showed
a significant (p < 0.02) lower content of total glutathione and protein sulfhydryls, and higher levels of oxidized glutathione (p < 0.03), malondialdehyde, and protein carbonyls (p < 0.05). Ethanol-intoxicated acinar cells showed a lower baseline amylase output compared to controls, with the difference
being significantly exacerbated by cerulein stimulation. After cerulein, the release of protein carbonyls by ethanol-treated
cells was significantly increased, whereas that of protein sulfhydryls was significantly decreased. In conclusion, ethanol
oxidatively damages pancreatic acinar cells; cerulein stimulation is followed by a lower output of amylase and by a higher
release of oxidized proteins by pancreatic acinar cells from ethanol-treated rats. These findings may account for the decreased
exocrine function, intraductular plug formation, and protein precipitation in alcoholic pancreatitis. |
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Keywords: | Amylase Cerulein Ethanol Glutathione Lipid peroxidation Pancreatic acini Protein carbonyls Protein oxidation Protein sulfhydryls |
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