Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine |
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Authors: | Rozalski Rafal Siomek Agnieszka Gackowski Daniel Foksinski Marek Gran Christine Klungland Arne Olinski Ryszard |
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Affiliation: | a Department of Clinical Biochemistry The Ludwik Rydygier Medical University in Bydgoszcz Karlowicza 24 85-092 Bydgoszcz Poland.b Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology University of Oslo, Rikshospitalet 0027 Oslo Norway. |
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Abstract: | In order to eliminate the possibility that diet may influence urinary oxidative DNA lesion levels, in our experiments we used a recently developed technique involving HPLC pre-purification followed by gas chromatography with isotope dilution mass spectrometric detection. This methodology was applied for the determination of the lesions: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 5-(hydroxymethyl)uracil (5HMUra) in the urine of mice fed with nucleic acid free diet and normal, unrestricted diet. The mean levels of 8-oxoGua, 8-oxodGuo and 5HMUra of the animals fed the normal diet reached the mean values of [Formula: See Text], [Formula: See Text] and [Formula: See Text] After feeding the mice for 12 days with nucleic acid free diet the respective values were [Formula: See Text], [Formula: See Text] and [Formula: See Text] respectively. The results clearly demonstrate that irrespective of the diet, the excretion rates were not statistically different during the course of feeding. The respective p values for the differences between lesions in the two types of diets were: 0.13 (8-oxoGua), 0.16 (8-oxodGuo), 0.18 (5-HMUra). Our results clearly indicate that diet does not contribute to urinary excretion of the lesions in mouse model. |
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Keywords: | Diet 8-oxoGua Oxidative DNA damage Urine |
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