A method for the rapid production of fine plant cell suspension cultures |
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| Authors: | P D Williams A K Wilkinson J A Lewis G M Black F Mavituna |
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| Institution: | (1) Department of Chemical Engineering, UMIST, Sackville Street, P.O. Box 88, M60 1QD Manchester, United Kingdom;(2) Biotechnology Department, Glaxo Group Research, Greenford Road, UB60HE Middlesex, United Kingdom;(3) Division of Chemical and Wood Technology, C.S.I.R.O., Private Bag 10, 3168 Clayton, Victoria, Australia |
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| Abstract: | A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effective in rapidly producing suspension cultures from callus cultures. The suspension cultures so produced are particularly useful for immobilisation, such as in porous polyurethane foam matrices. |
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