N.m.r. studies of the disulphated disaccharide obtained by degradation of bovine lung heparin with nitrous acid |
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Authors: | T N Huckerby P N Sanderson I A Nieduszynski |
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Affiliation: | 1. Nemours/Alfred I. duPont Hospital for Children, Wilmington, DE, USA;2. Department of Biological Sciences, University of Delaware, Newark, DE, USA;3. Department of Biochemistry, Shimane University, Shimane, Japan;4. Department of Pediatrics, Shimane University, Shimane, Japan;5. Department of Pediatrics, Gifu University, Gifu, Japan |
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Abstract: | The disulphated disaccharide IdoA(2SO3)-anManOH(6SO3) was prepared from bovine lung heparin by treatment with nitrous acid followed by borohydride reduction. The 1H- (400 MHz) and 13C-n.m.r. (100 MHz) spectra of this disaccharide derivative have been assigned completely using homonuclear spin-decoupling experiments, 13C-1H correlations, and a COSY-45 two-dimensional homonuclear correlation experiment. The 3JH,H values show that the IdoA(2SO3) residue exists in a single conformation throughout the temperature range 20-90 degrees. |
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