Isolation ofTricholoma matsutake andT. bakamatsutake cultures from field-collected ectomycorrhizas |
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| Authors: | Akiyoshi Yamada Takeo Ogura Yosuke Degawa Masatake Ohmasa |
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| Affiliation: | (1) Ibaraki Prefectural Forestry Center, Naka-machi, Naka-gun, 311-0122 Ibaraki, Japan;(2) Kanagawa Prefectural Museum of Natural History, 250-0031 Odawara-shi, Kanagawa, Japan;(3) Faculty of Agriculture, Shinshu University, Minami-minowa-mura, Kamiina-gun, 399-4598 Nagano, Japan;(4) Present address: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa-mura, Kamiina-gun, 399-4598 Nagano, Japan |
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| Abstract: | Tricholoma matsutake was isolated into pure cultures from field samples of ectomycorrhizas onPinus densiflora. The mycorrhizal tips were collected at different times of the year from a colony ofT. matsutake in aP. densiflora stand. The mycorrhizal tips were continuously washed with sterilized distilled water and diluted Tween 80 solution, surface-sterilized with calcium hypochlorite solution, and inoculated on several kinds of nutrient agar media. Most of the mycorrhizal tips collected in winter and spring produced colonies that were morphologically similar to cultures ofT. matsutake isolated from basidiocarps. The identity of isolates obtained from mycorrhizas was further confirmed to beT. matsutake based on fungal morphology and RFLP patterns of PCR amplified rDNA. The feasibility ofT. bakamatsutake isolation into pure culture from ectomycorrhizas onQuercus serrata was also confirmed. These results indicated that mycelium of matsutake mushrooms can be isolated into pure culture from ectomycorrhizas at different times of the year. Mycorrhizas of bothT. matsutake andT. bakamatsutake were not observed to have any specific association with soil fungi such asMortierella spp. |
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| Keywords: | basidiomycetes DNA identification Matsutake mushroom Mortierella soil microorganisms |
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