Sampling of solid spent agar media for gas chromatography of diffused acidic fermentation products |
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Authors: | Wilfred R Moorer |
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Institution: | University of Amsterdam, Department of Cariology, Endodontology and Pedodontology, Louwesweg 1, 1066 EA Amsterdam, The Netherlands |
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Abstract: | Abstract Sampling and processing of solid agar media for gas chromatographic analysis is described. Small pieces of agar are cut from a 'sterile' area of the plate leaving the actual bacterial growth undisturbed for further tests. It is shown that diffused fermentation acids are adequately represented in these samples to permit their use for phenotypic and taxonomic characterization of isolates. Because of the established qualitative and quantitative correspondence of fermentation patterns from agar-grown and from broth-grown bacteria, respectively, the method is offered as a rapid alternative for the sampling of spent culture broths. In addition, the sampling of spent agar plugs seems to hold promise for the study of defined mixed cultures and of mixed growth from primary plates. |
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Keywords: | Gas chromatography fermentation acids sampling technique blood agar |
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