Altered levels of the Taraxacum kok-saghyz (Russian dandelion) small rubber particle protein,TkSRPP3, result in qualitative and quantitative changes in rubber metabolism |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA;2. Nevada Proteomics Center, University of Nevada, Reno, NV 89557, USA;3. Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA;4. The Ohio State University, Department of Horticulture and Crop Science, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA;5. The Ohio State University, Department of Food, Agricultural and Biological Engineering, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA;1. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, PR China;2. State Key Laboratory Incubation Base, Danzhou, Hainan, PR China;3. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, Hainan, PR China;1. CNRS, LCPO, UMR 5629, F-33600 Pessac, France;2. Univ. Bordeaux, LCPO, UMR 5629, F-33600 Pessac, France;3. CNRS, CBMN, UMR 5248, F-33600 Pessac, France;4. Univ. Bordeaux, CBMN, UMR 5248, F-33600 Pessac, France;1. Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan;2. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan;3. Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan;4. Research and Development Institute, Thaksin University, Songkhla, Thailand;5. Department of Biochemistry, Mahidol University, Bangkok, Thailand;6. Department of Biochemistry, Prince of Songkla University, Hat-Yai, Thailand;1. Department of Food, Agricultural and Biological Engineering, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA;2. Department of Horticulture and Crop Science, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA;1. Department of Animal Sciences, The Ohio State University and Ohio Agricultural Research and Development Center (OARDC), Wooster, OH 44691, United States;2. Department of Food, Agricultural and Biological Engineering, The Ohio State University and Ohio Agricultural Research and Development Center (OARDC), Wooster, OH 44691, United States;3. Department of Horticulture & Crop Sciences, The Ohio State University and Ohio Agricultural Research and Development Center (OARDC), Wooster, OH 44691, United States |
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| Abstract: | Several proteins have been identified and implicated in natural rubber biosynthesis, one of which, the small rubber particle protein (SRPP), was originally identified in Hevea brasiliensis as an abundant protein associated with cytosolic vesicles known as rubber particles. While previous in vitro studies suggest that SRPP plays a role in rubber biosynthesis, in vivo evidence is lacking to support this hypothesis. To address this issue, a transgene approach was taken in Taraxacum kok-saghyz (Russian dandelion or Tk) to determine if altered SRPP levels would influence rubber biosynthesis. Three dandelion SRPPs were found to be highly abundant on dandelion rubber particles. The most abundant particle associated SRPP, TkSRPP3, showed temporal and spatial patterns of expression consistent with patterns of natural rubber accumulation in dandelion. To confirm its role in rubber biosynthesis, TkSRPP3 expression was altered in Russian dandelion using over-expression and RNAi methods. While TkSRPP3 over-expressing lines had slightly higher levels of rubber in their roots, relative to the control, TkSRPP3 RNAi lines showed significant decreases in root rubber content and produced dramatically lower molecular weight rubber than the control line. Not only do results here provide in vivo evidence of TkSRPP proteins affecting the amount of rubber in dandelion root, but they also suggest a function in regulating the molecular weight of the cis-1, 4-polyisoprene polymer. |
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