Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation system in Janibacter sp. TYM3221 |
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| Institution: | 1. Hainan Provincial Key Lab of R&D on Tropic Herbs, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China;2. School of Pharmacy, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China;3. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, PR China;1. Laboratory of Toxicology, School of Veterinary Medicine, Kitasato University, 35-1 Higashi 23-bancho, Towada-shi, Aomori 034-8628, Japan;2. Center for Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan |
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| Abstract: | Bacterial degradation of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) has been previously reported, however, its degradation enzyme system has not been characterized. In this study, a DDE-degrading bacterium, Janibacter sp. TYM3221, was isolated and characterized. Transformation of DDE was demonstrated by TYM3211 resting cells grown in LB in the presence and absence of biphenyl. Gas chromatography–mass spectrometry analysis revealed five metabolites of DDE containing a meta-ring cleavage product and 4-chlorobenzoic acid, suggesting that TYM3221 degrades DDE to 4-chlorobenzoic acid via a meta-ring cleavage product. A gene cluster, bphAaAbAcAd, which codes for biphenyl dioxygenase subunits, was cloned from TYM3221. A mutant strain with a bphAa-gene inactivation did not grow on biphenyl, and showed no DDE degradation activity. These results indicate that in strain TYM3221, the bphAa-coded biphenyl dioxygenase is involved not only in the metabolism of biphenyl but also in the degradation of DDE. |
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