J blood group active lipoproteins extracted from bovine erythrocytes |
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Authors: | Bruno Kirchhof Otto Wolfgang Thiele Maria Elisabeth Petri Johannes Koch |
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Affiliation: | Physiologisch-Chemisches Institut and Tierarztliches Institut der Universitat, Göttingen, West Germany |
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Abstract: | Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with our finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay. |
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