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Characterization of xanthans from selected Xanthomonas strains cultivated under constant dissolved oxygen |
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| Authors: | Sa´nchez A. Rami´rez M.E. Torres L.G. Galindo E. |
| Affiliation: | (1) Depto. de Bioingenieri´a, Instituto de Biotecnologi´a, Universidad Nacional Auto´noma de Me´xico, Apdo. Post. 510-3, Cuernavaca, Morelos, 62250, Me´xico;(2) Instituto de Ecologi´a, Apdo. Postal 63, Xalapa, Veracruz, 91000, Me´xico;(3) Coordinacio´n de Ingenieri´a Ambiental, Instituto de Ingenieri´a, UNAM, Ciudad Universitaria, Apdo. Postal 70-472, Coyoaca´n Me´xico, 04510, D.F. Me´xico |
| Abstract: | Seventeen wild-type Xanthomonas isolates were screened in terms of broth viscosity in shake-flasks. As culture conditions affect polymer characteristics, a fair comparison among isolates required their cultivation in a fermenter under controlled dissolved oxygen tension. Three isolates and a reference strain were studied. The mean molecular weights and molecular weight distributions of their xanthans were determined. Products showed different pyruvate (0.2–7%), acetate (5–10%) and proteinaceous nitrogen (1–3%) contents. The selected isolates exhibit properties which could improve xanthan gum production and some could be used to produce polymers with specific characteristics. |
| Keywords: | Dissolved oxygen screening xanthan Xanthomonas campestris |
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