Identification of DNA amplification fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.) |
| |
| Authors: | A E Men A Y Borisov S M Rozov K V Ushakov V E Tsyganov I A Tikhonovich P M Gresshoff |
| |
| Institution: | (1) Plant Molecular Genetics and Center for Legume Research, The University of Tennessee, 269 Ellington Plant Science Building, P.O. Box 1071, Knoxville, TN 37901, USA Fax: +1-423-974-2765 E-mail: pgresshoff@soybean.ag.utk.edu, US;(2) All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chosse 3, Saint-Petersburg, Pushkin 8, 189620, Russia, RU;(3) Institute of Cytology and Genetics, 630090, Russia, RU |
| |
| Abstract: | We demonstrate efficient genome mapping through a combination of bulked segregant analysis (BSA) with DNA amplification fingerprinting
(DAF). Two sets of 64 octamer DAF primers, along with two PCR programs of low- and high-annealing temperatures (30°C and 55°C,
respectively), appeared to be enough to locate molecular markers within 2–5 cM of a gene of interest. This approach allowed
the rapid identification of four BSA markers linked to the pea (Pisum sativum L.) Sym31 gene, which is responsible for bacteroid and symbiosome differentiation. Three of these markers are shown to be tightly linked
to the sym31 mutation. Two markers flanking the Sym31 gene, A21-310 and B1-277, cover a 4–5 cM interval of pea linkage group 3. Both markers were converted to sequence-characterized
amplified regions (SCARs). The flanking markers may be potential tools for marker-assisted selection or for positional cloning
of the Sym31 gene.
Received: 2 July 1998 / Accepted: 8 October 1998 |
| |
| Keywords: | DNA amplification fingerprinting Pea Symbiosis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
