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Hlad Immobilization in Polyacrylamide Gels
Authors:Robert Mertens   Guy L. Lemiere  Jozef A. Lepoivre  Frank C. Alderweireldt
Affiliation: a Antwerp University (RUCA), Laboratory of Chemical Technology, Antwerpen, Belgiumb Antwerp University (RUCA), Laboratory for Organic Chemistry, Antwerpen, Belgium
Abstract:The immobilization of horse liver alcohol dehydrogenase in a cross-linked acrylamide-N,N1-methylenebisacrylamide copolymer gel is described. The influence of monomer concentration, the degree of cross-linking and the polymerization technique on enzyme entrapment is studied. A bead-polymerization process produced the most useful and stable immobilized enzyme preparations.
Keywords:Horse liver alcohol dehydrogenase (HLAD)  Immobilization  Polyacrylamide gel
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