Hlad Immobilization in Polyacrylamide Gels |
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Authors: | Robert Mertens Guy L Lemiere Jozef A Lepoivre Frank C Alderweireldt |
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Institution: |
a Antwerp University (RUCA), Laboratory of Chemical Technology, Antwerpen, Belgium
b Antwerp University (RUCA), Laboratory for Organic Chemistry, Antwerpen, Belgium |
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Abstract: | The immobilization of horse liver alcohol dehydrogenase in a cross-linked acrylamide-N,N1-methylenebisacrylamide copolymer gel is described. The influence of monomer concentration, the degree of cross-linking and the polymerization technique on enzyme entrapment is studied. A bead-polymerization process produced the most useful and stable immobilized enzyme preparations. |
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Keywords: | Horse liver alcohol dehydrogenase (HLAD) Immobilization Polyacrylamide gel |
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