Lipase immobilized by adsorption |
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Authors: | Martin Norin John Boutelje Erland Holmberg Karl Hult |
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Institution: | (1) Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-10044 Stockholm, Sweden |
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Abstract: | Summary
Candida cylindraceae lipase was immobilized by adsorption to various hydrophilic and hydrophobic supports and studied with respect to the esterification rates of a primary and a secondary alcohol, respectively, in organic media. The reaction rates were compared with the rate of esterification with free lipase . When the secondary alcohol, (R, S)-1-phenylethanol, was used the highest reaction rates were measured for lipase adsorbed to the hydrophobic supports. When the primary alcohol, heptanol, was used free lipase exhibited the highest reaction rate. A kinetic explantation of these results is proposed. |
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