tRNA synthesis in germinating pollen |
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| Authors: | J. Süss J. Tupý |
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| Affiliation: | 1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo nám. 2, 0160 00, Praha 6, Czechoslovakia
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| Abstract: | The synthesis of tRNA was demonstrated in pollen ofNicotiana tabacum L. according to the incorporation of labeled uracil, adenosine and guanosine during 4 h of germination. tRNA was extracted from postribosomal supernatant and purified by polyacrylamide gel electrophoresis. The incorporation of guanosine was about 1.68 times higher than that of adenosine. This finding indicates that the whole tRNA chain is formed in pollen tubes. |
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