Oxidation of glutathione by peroxidase isoenzymes in fenugreek |
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| Authors: | D Balasimha M N Tewari |
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| Institution: | 1. Plant Biochemistry Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India
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| Abstract: | During the germination of fenugreek (Trigonella foenum graecum L.) sulfhydryl groups rapidly declined in cotyledon and seedling axis, while peroxidase activity increased. Studies on purified isoenzymes showed that GSH was oxidized by the isoenzymes and was accomplished in presence of cofactors, Mn2+ and DCP along with H2O2 (0.01 mM). This reaction was found to be peroxidatic in nature. The oxidation was inhibited by catechol but was enhanced by malic acid. |
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