The use of a multichannel scaling method to detect radioactive species in spread monolayers |
| |
Authors: | F H Tsao T K Foo P M DeLuca G Zografi |
| |
Institution: | Department of Pediatrics, University of Wisconsin, Madison 53706. |
| |
Abstract: | This study describes a simple and inexpensive method for monitoring radioactive species spread as monolayers at the air/water interface. The combination of a discriminator and multichannel scalar counter with a personal computer functions to unify all measurements, to simplify the operational process and data acquisition, and to provide a real-time display of the data. Its use is demonstrated by following the hydrolysis of L-alpha-1-14C]dioleoyl phosphatidylcholine by the enzyme, phospholipase A2, isolated from the porcine pancreas. |
| |
Keywords: | |
|
|