Characterization of the cyrl-2 UGA mutation in Saccharomyces cerevisiae |
| |
Authors: | Takashi Morishita Akira Matsuura and Isao Uno |
| |
Institution: | (1) Institute of Applied Microbiology, University of Tokyo, 113 Bunkyo-ku, Tokyo, Japan;(2) R and D Laboratories 1, Nippon Steel Corporation, 211 Kawasaki, Japan;(3) Present address: Department of Biology, Faculty of Science, University of Tokyo, 113 Bunkyo-ku, Tokyo, Japan |
| |
Abstract: | Summary
cyrl-2 is a temperature-sensitive mutation of the yeast adenylate cyclase structural gene, CYR1. The cyrl-2 mutation has been suggested to be a UGA mutation since a UGA suppressor SUP201 has been isolated as a suppressor of the cyrl-2 mutation. Construction of chimeric genes restricted the region containing the cyrl-2 mutation, and the cyrl-2 UGA mutation was identified at codon 1282, which lies upstream of the region coding for the catalytic domain of adenylate cyclase. Alterations in the region upstream of the cyrl-2 mutation site result in null mutations. The complete open reading frame of the cyrl-2 gene expressed under the control of the GAL1 promoter complemented cyrl-dl in a galactose-dependent manner. These results suggest that at the permissive temperature weak readthrough occurs at the cyrl-2 mutation site to produce low levels of active adenylate cyclase. An endogenous suppressor in yeast cells is assumed to be responsible for this readthrough. |
| |
Keywords: | Saccharomyces cerevisiae cyrl-2 Nonsense mutation CAMP |
本文献已被 SpringerLink 等数据库收录! |
|