Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli. |
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Authors: | W Ebel G J Vaughn H K Peters rd and J E Trempy |
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Institution: | W Ebel, G J Vaughn, H K Peters, 3rd, and J E Trempy |
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Abstract: | Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon+ cells, increased 38-fold in lon+ cells carrying a Tn10::delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::delta kan insertion. Sequencing of both sides of the Tn10::delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane-derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed. |
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