A mimotope of Pre-S_2 region of surface antigen of viral hepatitis B screened by phage display |
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Authors: | WU YU ZHANG YING WAN DAO GUO LI YAN TANG WEI ZHOU Institute of Immunology PLA The Third Medical University Chongqing China Medical Center General Hospital of Weifang Weifang China |
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Affiliation: | WU YU ZHANG,YING WAN,DAO GUO LI,YAN TANG,WEI ZHOU Institute of Immunology,PLA,The Third Medical University,Chongqing 400038,China Medical Center,General Hospital of Weifang,Weifang 26100,China |
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Abstract: | To acquire the phage-displayed mimotopes which mimic the specificity of hepatitis B virus surface antigen (HBsAg), a random peptide library expressing linear peptide with 12 amino asids in length were used to screen with the serum from a hepatitis B virus infected patient in the recovery phase. After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33. Two phagotopes were identified and one of them was confirmed as mimotope by competition experiment. Based on the mimotpe, a multiple antigenic peptide with four branches was synthesized by solid phase peptide synthesis. The antiginicity and specificity of the synthesized antigen was tested in BALB/c mice compared with the native epitope-based antigen. The results showed that the mimotope-based antigen could evoke higher titer of antibodies with the same specificity of the epitope-based antigen. Those findings indicate mimotopes can be used in antigen and vaccine design. |
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Keywords: | Mimotope hepatitis B virus raccine antibody |
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