| Cloning and characterization of a novel gene（C17orf25）from the deletion region on chromosome 17p13.3 in hepatocelular carcinoma |
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| 引用本文： | Qin WX,Wan F,Sun FY,Zhang PP,Han LW,Huang Y,Jiang HQ,Zhao XT,He M,Ye Y,Cong WM,Wu MC,Zhang LS,Yang NW,Gu JR. Cloning and characterization of a novel gene（C17orf25）from the deletion region on chromosome 17p13.3 in hepatocelular carcinoma[J]. Cell research, 2001, 11(3): 209-216 |
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| 作者姓名： | Qin WX Wan F Sun FY Zhang PP Han LW Huang Y Jiang HQ Zhao XT He M Ye Y Cong WM Wu MC Zhang LS Yang NW Gu JR |
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| 作者单位： | [1]NationalLaboratoryforOncogenesandRelatedGenes,ShanghaiCancerInstitute,25／Ln2200XieTuRoad,Shanghai200032,China [2]NationalLaboratoryforOncogenesandRelatedGenes,ShanghaiCancerInstitute,25／Ln2200XieTuRoad,S |
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| 基金项目： | the National 863High Technology Research and Development Pro-gram of China (Zl9-02--0l-0l) to Wan DF and theProject of Ch |
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| 摘 要： | INTRODUCTIONLoss of heterozygosity (LOH) at chromosoma1loci associated with tumor suppressor genes has beenimplicated in the genesis of many types of humanmalignancies. On the basis of frequent LOH in tu-mors, coupled with linkage analysis in some heredi-tary cancer syndromes, a number of tumor suppres-sor genes, such as RB[l], DCC[2], NF2[3], VHLI4],MTh1[5], DML/OM1[6], and PrsN/rmC1[7l have been successively isolated.It has beell reported that LOH occurred at l7p invarious ty…
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| 关 键 词： | 肝细胞癌 染色体17β13.3 杂合性铁 转染 C17orf25基因 |
Cloning and characterization of a novel gene (C17orf25) from the deletion region on chromosome 17p13.3 in hepatocelular carcinoma |
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| Qin W X,Wan F,Sun F Y,Zhang P P,Han L W,Huang Y,Jiang H Q,Zhao X T,He M,Ye Y,Cong W M,Wu M C,Zhang L S,Yang N W,Gu J R. Cloning and characterization of a novel gene (C17orf25) from the deletion region on chromosome 17p13.3 in hepatocelular carcinoma[J]. Cell research, 2001, 11(3): 209-216 |
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| Authors: | Qin W X Wan F Sun F Y Zhang P P Han L W Huang Y Jiang H Q Zhao X T He M Ye Y Cong W M Wu M C Zhang L S Yang N W Gu J R |
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| Affiliation: | National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, China. |
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| Abstract: | Using a combination of hybridization of PAC to a cDNA library and RACE technique, we isolated a novel cDNA, designated as C17orf25 (Chromosome 17 open rea(ling frame 25, previously named it HC71A), from the deletion region on chromosome 17p13.3. The cDNA encodes a protein of 313 amino acids with a calculated molecular mass of 34.8 kDa. C17orf25 is divided into 10 exons and 9 introns, spanning 23 kb of genomic DNA. Northern blot analysis showed that the mRNA expression of C17orf25 was decreased in hepatocellular carcinoma samples as compared to adjacent noncancerous liver tissues from the same patients. The transfection of C17or25 into the hepatocellular carcinoma cell SMMC7721 and overexpression could inhibit the cell growth. The above results indicate that C17orf25 is a novel human gene, and the cloning and preliminary characterization of C17orf25 is a prerequisite for further functional analysis of this novel gene in human hepatocellular carcinoma. |
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| Keywords: | Chromosome 17p13.3 1lss of heterozygosity hepatocellular carcinoma transfection novel human gene (C17orf25) |
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