Id2 reverses cell cycle arrest induced by {gamma}-irradiation in human HaCaT keratinocytes |
| |
Authors: | Baghdoyan Sandrine Lamartine Jérôme Castel David Pitaval Amandine Roupioz Yoann Franco Noreli Duarte Mariela Martin Michèle T Gidrol Xavier |
| |
Institution: | Service de Génomique Fonctionnelle, Commissariat à l'Energie Atomique-Evry, 2 rue Gaston Crémieux, CP22, 91057 Evry, France. |
| |
Abstract: | Id2 plays a key role in epithelial cells, regulating differentiation, the cell cycle, and proliferation. Because human skin constantly renews itself and is the first target of irradiation, it is of primary interest to evaluate whether such a gene may be regulated in keratinocytes exposed to ionizing radiation. We show here that Id2 is induced in response to gamma-irradiation and have investigated the consequence of this regulation on cell fate. Using RNA interference, we observed that Id2 extinction significantly reduces cell growth in human keratinocytes through the control of the G(1)-S transition of the cell cycle. We have investigated whether the impact of Id2 on the cell cycle may have a physiological role on the cell's ability to cope with radiative stress. Indeed, when Id2 is down-regulated through interfering RNA, cells are more sensitive to irradiation. Conversely, when Id2 is overexpressed, this somehow protects the cell. We propose that Id2 favors reentering the cell cycle after radiation-induced cell cycle arrest to permit the recovery of keratinocytes exposed to ionizing radiation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|