Non-antisense cellular responses to oligonucleotides |
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Authors: | Alain Anselmet, Ebrahim Mayat, Stefan Wietek, Paul G Layer, Bernard Payrastre,Jean Massouli |
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Affiliation: | CNRS UMR 8544, Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France. anselmet@biologie.ens.fr |
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Abstract: | Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10–20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments. |
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Keywords: | Antisense oligonucleotide Double strand Cell aggregation G-quartet Neurite Platelet |
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