Affinity Purification of Human τ Proteins and the Construction of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay for Human τ Detection |
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Authors: | Marc Mercken&dagger ,Mark Vandermeeren&dagger ,Ursula Lü bke&Dagger ,Jan Six,Jef Boons&dagger ,Eugè ne Vanmechelen,re Van De,Voorde,Jan Gheuens&dagger |
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Affiliation: | Innogenetics, Ghent, Belgium. |
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Abstract: | Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml. |
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Keywords: | τ protein Monoclonal antibodies Alzheimer's disease Enzyme-linked immunosorbent assay Affinity purification |
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