A direct radioimmunoassay for 5 alpha-androstane-3 alpha,17 beta-diol 17-glucuronide. |
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| Authors: | P N Rao A M Rodriguez P H Moore J W Cessac |
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| Affiliation: | Department of Organic Chemistry, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147. |
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| Abstract: | Synthesis of the 11 alpha-hemiglutaryl derivative of 5 alpha-androstane-3 alpha,17 beta-diol 17-glucuronide (androstane-diol-17G) starting from androsta-4,9(11)-diene-3,17-dione through a 10-step sequence and the preparation of its bovine serum albumin conjugate is described. By using this conjugate, antiserum was raised in rabbits which proved to be very specific for androstanediol-17G. A direct radioimmunoassay using a double antibody procedure is described for the measurement of androstanediol-17G from plasma without prior chromatography. |
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