Characterization of Cryptococcus neoformans by random DNA amplification |
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| Authors: | P Ruma SCA Chen TC Sorrel AG Brownlee |
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| Institution: | The Centre for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Westmead, NSW;Divison of Animal Production, CSIRO, Prospect NSW, Australla |
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| Abstract: | Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neofirmans . The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans , and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neofirmans var. gattii , although its two serotypes, B and C, were only differentiated with one primer combination out of seven. |
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