| 用傅里叶变换红外光谱技术研究HIV-1融合肽的插膜深度与角度 |
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| 引用本文： | 邱阳,林克椿,聂松青. 用傅里叶变换红外光谱技术研究HIV-1融合肽的插膜深度与角度[J]. 生物物理学报, 2001, 17(1): 46-52 |
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| 作者姓名： | 邱阳 林克椿 聂松青 |
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| 作者单位： | 北京大学医学部生物物理系, |
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| 基金项目： | 国家自然科学基金39770181资助 |
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| 摘 要： | 为探讨HIV－1gp41N端融合肽诱导膜融合的机理，利用傅里叶变换红外光谱技术研究了化学方法合成的代表HIV－1gp41N末端的23肽（HIV
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| 关 键 词： | HIV－1融合肽 FTIR H／D交换 分子取向 人类免疫缺陷病毒Ⅰ型 插膜深度 傅里叶变换红外光谱 |
| 文章编号： | 1000－6737（2001）01－0046－07 |
| 修稿时间： | 2000-06-20 |
STUDY ON THE MEMBRANE INSERTION DEPTH AND ORIENTATION OFHIV-1 FUSION PEPTIDE BY FOURIER TRANSFORMEDINFRARED SPECTORSCOPY |
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| QIU Yang,LIN Ke-chun,NIE Song-qing. STUDY ON THE MEMBRANE INSERTION DEPTH AND ORIENTATION OFHIV-1 FUSION PEPTIDE BY FOURIER TRANSFORMEDINFRARED SPECTORSCOPY[J]. Acta Biophysica Sinica, 2001, 17(1): 46-52 |
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| Authors: | QIU Yang LIN Ke-chun NIE Song-qing |
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| Abstract: | To investigate the mechanism of membrane fusion induced by HIV-1 gp41 N terminal fusion peptide, we studied its membrane insertion state and orientation by H/D exchange and polarized FTIR in acidic POPG and neutral POPC liposomes, respectively. The results indicate that only 11.9% of backbone amide protons of HIVWT in POPG vesicles undergo H/D exchange, whereas 44.6% amide protons are exchangeable for HIVWT in POPC SUV. The average angles of α-helix and β-strand of HIVWT with respect to the POPG bilayer surface are 29±2° and 25±1°, respectively. In the presence of POPC SUV, the average angle between the α-helix of HIVWT and the bilayer surface is 22±1°, and the average angle of β-strand of HIVWT is 26±3°. The membrane insertion state of HIVWT was discussed. |
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| Keywords: | HIV-1 fusion peptide FTIR H/D exchange Orientation |
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