| 洋葱叶肉原生质体培养再生小植株 |
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| 引用本文： | 王光远,夏镇澳,王六发. 洋葱叶肉原生质体培养再生小植株[J]. 分子细胞生物学报, 1986, 0(4) |
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| 作者姓名： | 王光远 夏镇澳 王六发 |
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| 作者单位： | 中国科学院上海植物生理研究所(王光远，夏镇澳)，中国科学院上海植物生理研究所(王六发) |
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| 摘 要： | 用纤维素酶(EA 3-867)和离析酶(Macerozyme R-10)酶解洋葱叶肉细胞,游离获得大量具有活力的原生质体。在MS培养基上(附加2,4-D 2,6-BA 0.5 mg/l,原生质体能再生细胞壁,生长,分裂,形成类似球形的愈伤组织;将它们分别移入分化培养基MS_1,MS_2,MS_3,得到再生的小植株。
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REGENERATED PLANTLETS FROM CULTURED MESOPHYLL PROTOPLASTS OF ONION (ALLIUM CEPA L.) |
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| Wang Guang-yuan,Xia Zhen-ao. REGENERATED PLANTLETS FROM CULTURED MESOPHYLL PROTOPLASTS OF ONION (ALLIUM CEPA L.)[J]. Journal of Molecular Cell Biology, 1986, 0(4) |
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| Authors: | Wang Guang-yuan Xia Zhen-ao |
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| Abstract: | Large amount of viable mesophyllprotoplasts were isolated enzymaticallyfrom onion(Allium cepa L.).The pro-toplasts grew and divided when culturedin thin liquid layers of MS mediumwith 2,4-D 2,6 BA 0.5 mg/1,mannitol0.5 M,sucrose 2%.Calli were subcultu-red on MS medium without mannitol.After transferring to modified MS me-dium(MS_1,MS_2,MS_3)calli regeneratedbuds or bulblets and regenerated plant-lets had been obtained. |
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| Keywords: | Protoplast.Callus.Plantlet. |
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