Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium |
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Authors: | G Ooms M M Burrell A Karp M Bevan J Hille |
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Institution: | (1) Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, UK;(2) Department of Molecular Genetics, Plant Breeding Institute, Maris Lane, CB2 2LQ Trumpington, Cambridge, UK;(3) Department of Plant Molecular Biology, University of Leiden, Wassenaarseweg 66, NL-2333 AL Leiden, The Netherlands;(4) Present address: Department of Molecular Biology, Agricultural University of Wageningen, De Dreyen 11, NL-6703 BC Wageningen, The Netherlands |
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Abstract: | Summary Derivatives of potato (Solanum tuberosum cv.'s Maris Bard and Desiree ) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four Maris Bard plants transformed with pRAL1834 T-DNA and two Desiree plants with pBIN6 T-DNA. The transformed Maris Bard plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed Desiree plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin. |
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Keywords: | Genetic manipulation Neomycin phosphotransferase Mixed infection Somaclonal variation Solanum tuberosum |
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