Anaerobic hydrolysis and acidification of organic substrates: determination of anaerobic hydrolytic potential |
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Authors: | Rajagopal Rajinikanth Béline Fabrice |
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Institution: | Cemagref, UR GERE, 17, Avenue de Cucillé, CS 64427, F-35044 Rennes, France Université Européenne de Bretagne, France |
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Abstract: | This study aimed to develop a biochemical-test mainly to evaluate the hydrolytic-potential of different substrates and to apply this test to characterize various organic substrates. Outcome of this study can be used for optimization of the WWTPs through enhancement of N/P removal or anaerobic digestion. Out of four series of batch experiments, the first two tests were conducted to determine the optimal operating conditions (test duration, inoculum-ratio etc.) for the hydrolytic-potential test using secondary and synthetically-prepared sludges. Based on the results (generation of CODs, pH and VFA), test duration was fixed between 6 and 7 d allowing to attain maximum hydrolysis and to avoid methanogenesis. Effect of inoculum-ratios on acid fermentation of sludge was not significantly noticed. Using this methodology, third and fourth tests were performed to characterize various organic substrates namely secondary, pre-treated sludge, pig and two different cattle slurries. VFA production was shown to be substantially dependent on substrates types. |
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Keywords: | Hydrolysis Acidification Fermentation time Inoculum ratio Volatile fatty acids (VFAs) |
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